Fig. 5

Overexpressed NEU1 reversed the effects of upregulated miR-23b-3p on inflammatory cytokines levels and NEU1 expression in obese mice. a-d Levels of inflammatory cytokines CRP (a), IL-6 (b), TNF-α (c) and MCP1 (d) after obesity-induced IR model construction and injection of lentivirus carriers for miR-23b-3p mimic and NEU1 overexpression plasmid were detected with ELISA. e-f NEU1 protein/GAPDH expressions after obesity-induced IR model construction and injection of lentivirus carriers for miR-23b-3p mimic and NEU1 overexpression plasmid were measured with Western blot. GAPDH was employed as internal control. All experiments have been performed in triplicate and data were expressed as mean ± standard deviation (SD). ^**P < 0.01, ^***P < 0.001, vs. Control; ^{^}P < 0.05, ^{^^}P < 0.01, ^{^^^}P < 0.001, vs. MC; ^##P < 0.01, ^###P < 0.001, vs. NC; ^ΔP < 0.05, ^ΔΔP < 0.01, ^ΔΔΔP < 0.001, vs. M; ^ξξP < 0.01, ^ξξξP < 0.001, vs. NEU1